
	Pakistan Journal of Biological Sciences
	Year: 2008 \| Volume: 11 \| Issue: 22 \| Page No.: 2553-2559 DOI: 10.3923/pjbs.2008.2553.2559

Some Studies of α-Amylase Production Using Aspergillus oryzae

Z. Esfahanibolandbalaie, K. Rostami and S. S. Mirdamadi

Abstract: The extracellular α-amylase production by Aspergillus oryzae was studied in submerged fermentation using an Adlof-Kuhner orbital shaker. The effect of initial pH values in the range of 4 to 7.5 on enzyme production was investigated and initial pH medium of 6.2±0.1 resulted in enhanced α-amylase production. The effect of carbon and nitrogen source and composition was examined and it has been observed that corn starch concentration of 15 g L^-1 has sound effect on enzyme production. The medium containing corn starch, sodium nitrate resulted in considerable higher enzyme production. Further, the yeast extract of 2.5 g L^-1 in the medium produced higher enzyme in view to other organic nitrogen sources. The effect of temperature on α-amylase production from 20 to 40°C has been studied and at 35±1°C higher α-amylase has been obtained. The effect of shaker’s speed on α-amylase production from 50 to 200 rpm was investigated. And at about 180 rpm higher enzyme production has been observed. In the present study, it has been found that glucose has repressing effect on α-amylase production using A. oryzae PTCC5164.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Studies on Low-cost Substrates and Other Cultural Conditions for Optimal Amylase Yield from Aspergillus oryzae-SR2

How to cite this article:

Z. Esfahanibolandbalaie, K. Rostami and S. S. Mirdamadi, 2008. Some Studies of α-Amylase Production Using Aspergillus oryzae. Pakistan Journal of Biological Sciences, 11: 2553-2559.

DOI: 10.3923/pjbs.2008.2553.2559

URL: https://scialert.net/abstract/?doi=pjbs.2008.2553.2559

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		Thomson ISI

Science Alert