
	Pakistan Journal of Biological Sciences
	Year: 2008 \| Volume: 11 \| Issue: 20 \| Page No.: 2451-2453 DOI: 10.3923/pjbs.2008.2451.2453

Hesperidin from Citrus sinensis Cultivated in Dezful, Iran

N. Aghel, Z. Ramezani and S. Beiranvand

Abstract: This study described procedures for extracting and quantitation of hesperidin in the waste orange peel of Dezful. Two extracting procedure were used. In procedure A hesperidin was isolated from orange peel by extracting the dry peel first with petroleum ether, removing the essential oil and then with methanol. In procedure B alkaline extraction followed by acidification of the extract was used. It was purified by treatment with formamide-activated charcoal. Detailed analysis of UV, IR, ^1HNMR, ^13CNMR and Mass spectroscopic data confirm the structure and extent of purity of extracted hesperidin. The spectroscopic results of two extract showed that procedure A produced high extraction yield and more purified hesperidin. Pure hesperidin in gram quantity (11.7% for procedure A and 7.39% for procedure B) was obtained in one purification cycle.

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How to cite this article:

N. Aghel, Z. Ramezani and S. Beiranvand, 2008. Hesperidin from Citrus sinensis Cultivated in Dezful, Iran. Pakistan Journal of Biological Sciences, 11: 2451-2453.

DOI: 10.3923/pjbs.2008.2451.2453

URL: https://scialert.net/abstract/?doi=pjbs.2008.2451.2453

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