Submit Manuscript

Pakistan Journal of Biological Sciences

Year: 2008 | Volume: 11 | Issue: 12 | Page No.: 1541-1550
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 720

Search

Authors

Askary Mehry

Mostajeran Akbar

Emtiazi Giti

Keywords

Research Article

Colonization and Nitrogenase Activity of Triticum aestivum (cv. Baccross and Mahdavi) to the Dual Inoculation with Azospirillum brasilense and Rhizobium meliloti plus 2,4-D

Askary Mehry, Mostajeran Akbar and Emtiazi Giti
The potential enhancement of root colonization and nitrogenase activity of wheat cultivars (Baccross and Mahdavi) was studied with application of two Azospirillum brasilense strains (native and Sp7) co-inoculated with two Rhizobium meliloti strains (native and DSMZ 30135). The results indicated that the colonization was different due to the strains and cultivars of wheat were used. Native A. brasilense colonized wheat root better than Sp7 strain. However, Baccross cv. reacted better with native Azospirillum compared to Mahdavi cv. which reacted better with Sp7. When plants inoculated with dual inoculants (SP7 with standard Rhizobium), the colonization of Azospirillum were increased significantly (from 1.67x105 to 22x105 cfu g-1 FW for Baccras cv. and 3.67x105 to 26x105 cfu g-1 FW for Mahdavi cultivar). When the standard Rhizobium as co-inoculants changed to the native Rhizobium, the colonization of Azospirillum was higher when compared to the single inoculants but was almost the same when compared to the standard Rhizobium. When the standard or native strains of Rhizobium used as single inoculation of wheat roots, the number of Rhizobium in the wheat roots were not changed significantly. However, when plants co-inoculated with Rhizobium and Azospirillum, the colonization of Rhizobium was increased. Co-inoculation of standard strain of R. melilot with A. brasilense Sp7 showed that the colonization of Rhizobium were increased from 0.67x105 to 21x105 cfu g-1 FW for Baccross cv. and 0.33x105 to 18x105 cfu g-1 FW for Mahdavi cv. This behavior was the same when inoculation of Rhizobium was happened with the native one. In dual inoculation, the highest nitrogenase activity was measured in combination of the local strains (native A. brasilense with the native R. meliloti) and the lower one belongs to the combination of standard strains (Sp7 with standard R. meliloti). The difference in nirtogenase activity for different cultivars of wheat with Sp7 and standard Rhizobium is not significant but the difference for Sp7 strain plus native Rhizobium is significant (p>0.05). However, the differences were not significant (p<0.05) for nitrogenase activity in bacterial tubes, the difference for nitrogenase activity of co-inoculated plants with combination of Sp7 and Rhizobium either standard or native were significantly different
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *