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Pakistan Journal of Biological Sciences
  Year: 2008 | Volume: 11 | Issue: 9 | Page No.: 1246-1251
DOI: 10.3923/pjbs.2008.1246.1251
 
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Use of CLSA and SPME-Headspace Techniques Followed by GC-MS Analysis to Extract and Identify the Floral Odorants

Mourad Shonouda, Sergio Angeli, Stefan Schutz and Stefan Vidal

Abstract:
Flowers of Ziziphus spina christi are known to be attractive for parasitoids and predators. In Y-tube olfactometer experiments, the dried flowers attracted significantly (p<0.001) the female parasitoids Aphelinus abdominalis. The flower volatile compounds were analyzed to understand which compounds could be specifically responsible for this attractiveness. The volatile compounds of Ziziphus flowers were extracted by closed-loop-stripping-analysis (CLSA) and also by solid phase microextraction (SPME) followed by gas chromatography-mass spectrometry (GC-MS) analysis. The main chemical classes of the volatile compounds are aldehydes, monoterpene-alcohols, ketones and hydrocarbons. Flower extract and some specific compounds will be further tested for their responsiveness to predators and parasitoids in behavioural and electrophysiological experiments.
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How to cite this article:

Mourad Shonouda, Sergio Angeli, Stefan Schutz and Stefan Vidal, 2008. Use of CLSA and SPME-Headspace Techniques Followed by GC-MS Analysis to Extract and Identify the Floral Odorants. Pakistan Journal of Biological Sciences, 11: 1246-1251.

DOI: 10.3923/pjbs.2008.1246.1251

URL: https://scialert.net/abstract/?doi=pjbs.2008.1246.1251

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