Production and Characterization of a Mesophilic Lipase Isolated from Bacillus stearothermophilus AB-1


	Pakistan Journal of Biological Sciences
	Year: 2008 \| Volume: 11 \| Issue: 8 \| Page No.: 1100-1106 DOI: 10.3923/pjbs.2008.1100.1106

Production and Characterization of a Mesophilic Lipase Isolated from Bacillus stearothermophilus AB-1

Emad Abd El-moniem Abada

Abstract: Using Bacillus stearothermophilus AB-1 isolated from air, the production of lipase was attempted along with its purification and characterization studies. When different carbon and nitrogen sources were supplemented in the culture medium, xylose, tryptophan, alanine, phenylalanine and potassium nitrate were found to be the best. During cultivation, the strain secreted most of its lipase content after 48 h. In particular, the lipase produced in the culture broth showed 300 U mL^-1 when cultivated at optimal temperature and pH of 35 °C and 7.5, respectively. The enzyme was purified using 60% ammonium sulfate precipitation and sephadex G200 column chromatography. The enzyme was stable up to 40 °C and in the range of pH 7-8. This research reports for the first time the characterization of mesophilic lipase from Bacillus stearothermophilus AB-1 isolated from air.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Investigation of Isolated Lipase Producing Bacteria from Oil-contaminated Soil with Proteomic Analysis of its Proteins Responsive to Lipase Inducer

How to cite this article:

Emad Abd El-moniem Abada , 2008. Production and Characterization of a Mesophilic Lipase Isolated from Bacillus stearothermophilus AB-1. Pakistan Journal of Biological Sciences, 11: 1100-1106.

DOI: 10.3923/pjbs.2008.1100.1106

URL: https://scialert.net/abstract/?doi=pjbs.2008.1100.1106

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		Thomson ISI

Follow @scialert

Science Alert