Measurement of Within and Between Genetic Variability in Duck Breeds by RAPD Markers

Research Article

Measurement of Within and Between Genetic Variability in Duck Breeds by RAPD Markers

Mohsen Gholizadeh, Ghodrat Rahimi Mianji, Abbas Ghobadi, Hadi Sayyah Zadeh and Shahab Montazami

A total of 200 genomic DNAs were isolated from the four breeds of duck: Muscovy, Pekin, Khaki Campbell and Native, through a modified salting out procedure. The samples were used in a Polymerase Chain Reaction (PCR) with 27 RAPD markers. Amplified PCR-products with the markers were separated on a 2% agarose gel and stained with ethidium bromide. To evaluate the bands, polymorphic and monomorphic bands were described. Genetic similarity and genetic distance were calculated. The RAPD analysis data from 7 primers were utilized in estimating genetic similarity between four breeds, which ranged from 0.46 to 0.77 between the breeds. The maximum genetic distance was observed between Muscovy and Native duck breeds (0.76).

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Mohsen Gholizadeh, Ghodrat Rahimi Mianji, Abbas Ghobadi, Hadi Sayyah Zadeh and Shahab Montazami, 2007. Measurement of Within and Between Genetic Variability in Duck Breeds by RAPD Markers. Pakistan Journal of Biological Sciences, 10: 982-985.

DOI: 10.3923/pjbs.2007.982.985

URL: https://scialert.net/abstract/?doi=pjbs.2007.982.985

Pakistan Journal of Biological Sciences

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Authors

Mohsen Gholizadeh

Ghodrat Rahimi Mianji

Abbas Ghobadi

Hadi Sayyah Zadeh

Shahab Montazami

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Research Article

Measurement of Within and Between Genetic Variability in Duck Breeds by RAPD Markers

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