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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 6 | Page No.: 955-958
DOI: 10.3923/pjbs.2007.955.958
 
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Amplification and Cloning of Herpes Simplex Virus Type 2 Glycoprotein G from an Iranian Isolate

Hoorieh Soleimanjahi, Marzieh Jamalidoost, Fatemeh Fotouhi and Zahra Meshkat

Abstract:
Herpes Simplex Virus type-2 (HSV-2) is the main cause of genital herpes infection. Its prevalence is increasing worldwide and varies widely with generally higher rate in developing than developed countries and urban than rural areas. HSV-2 Iranian isolate was propagated in HeLa cell line. The viral genome was extracted by phenol-chloroform and used as template in nested polymerase chain reactions (n-PCR) to amplify gG-2 gene. The amplified gene was cloned into a cloning vector (pTZ57R/T) and transformed into competent E. coli DH5a. The recombinant vector encoding the gene of interest was confirmed by restriction enzyme analysis and sequencing.
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How to cite this article:

Hoorieh Soleimanjahi, Marzieh Jamalidoost, Fatemeh Fotouhi and Zahra Meshkat, 2007. Amplification and Cloning of Herpes Simplex Virus Type 2 Glycoprotein G from an Iranian Isolate. Pakistan Journal of Biological Sciences, 10: 955-958.

DOI: 10.3923/pjbs.2007.955.958

URL: https://scialert.net/abstract/?doi=pjbs.2007.955.958

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