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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 4 | Page No.: 659-663
DOI: 10.3923/pjbs.2007.659.663
 
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Somatic Embryogenesis and Plant Regeneration from Protoplast Culture of Crocus pallasii Subsp. haussknechtii

Roya Karamian

Abstract:
A protocol has been developed for plant regeneration from protoplast culture of Crocus pallasii subsp. haussknechtii using regenerable embryogenic calli obtained from shoot meristem culture on MS+9.28 μM kinetin+4.52 μM 2,4-D. Protoplasts were isolated directly from embryogenic calli, embedded in Ca-alginate beads and cultured with nurse cells in MS+4.64 μM kinetin+4.52 μM 2,4-D+5.68 μM ascorbic acid+0.3 M mannitol at 20±2°C in darkness. After appearing of microcalli on the surface of the beads, they were transferred onto 1/2MS+2.32 μM kinetin+2.26 μM 2,4-D+5.68 μM ascorbic acid for growth of embryogenic calli. Somatic embryos matured on MS medium growth regulator free and germinated on 1/2MS+14.45 μM GA3 +4.43 μM BA at 20±2°C in a 16/8 h light/dark cycle.
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How to cite this article:

Roya Karamian , 2007. Somatic Embryogenesis and Plant Regeneration from Protoplast Culture of Crocus pallasii Subsp. haussknechtii. Pakistan Journal of Biological Sciences, 10: 659-663.

DOI: 10.3923/pjbs.2007.659.663

URL: https://scialert.net/abstract/?doi=pjbs.2007.659.663

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