Species Identification and Sex Determination of the Genus Nepenthes (Nepenthaceae)


	Pakistan Journal of Biological Sciences
	Year: 2007 \| Volume: 10 \| Issue: 4 \| Page No.: 561-567 DOI: 10.3923/pjbs.2007.561.567

Species Identification and Sex Determination of the Genus Nepenthes (Nepenthaceae)

Runglawan Sudmoon, Tawatchai Tanee, Piya Mokkamul and Arunrat Chaveerach

Abstract: Nepenthes species are well known for their ornamentally attractive pitchers. The species diversity was randomly surveyed in some conservation areas of Thailand and three species were found, namely N. gracilis Korth., N. mirabilis Druce. and N. smilesii Hemsl. Young plants as unknown species from Chatuchak market were added in plant sampled set. Thirty two Inter Simple Sequence Repeat (ISSR) primers were screened and 13 successful primers were used to produce DNA banding patterns for constructing a dendrogram. The dendrogram is potentially power tool to identify unknown species from Chatuchak market, differentiate species population, population by geographical areas and sex determination. The geographical area of N. mirabilis was specified to Southern and Northeastern regions and finally, subdivided into exact areas according to province. Male and female plants of N. gracilis at Phu Wua Wildlife Sanctuary and N. mirabilis at Bung Khonglong non-hunting area were determined. Two unknown species from Chatuchak market were analyzed to be N. mirabilis with the genetic similarities (S) 77.2 to 84.7. Be more sex specific in all sample studied, 37 Random Amplified Polymorphic DNA (RAPD) primers were investigated. The result shows that only one RAPD primer show high resolution results at about 750 bp specific male-related marker.

[Fulltext PDF] [Fulltext HTML] [XML: Abstract + References] [References] [View Citation] [Report Citation]

How to cite this article:

Runglawan Sudmoon, Tawatchai Tanee, Piya Mokkamul and Arunrat Chaveerach, 2007. Species Identification and Sex Determination of the Genus Nepenthes (Nepenthaceae). Pakistan Journal of Biological Sciences, 10: 561-567.

DOI: 10.3923/pjbs.2007.561.567

URL: https://scialert.net/abstract/?doi=pjbs.2007.561.567

COMMENT ON THIS PAPER

	Full Name:
	E-mail:
	Comments:

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		AGRIS
		ASCI-Database
		Cambridge Scientific Abstract
		Chemical Abstract Services
		Google Scholar
		ISI Web of Knowledge
		PubMed
		SCIMAGO
		SCOPUS
		Thomson ISI