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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 3 | Page No.: 481-485
DOI: 10.3923/pjbs.2007.481.485
 
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Comparison of Somatic Embryogenesis in Medicago sativa and Medicago truncatula

F. Hoori, A.A. Ehsanpour and A. Mostajeran

Abstract:
In this study, the regeneration through embryogenesis of two species of Medicago were studied. Seeds of Medicago sativa cv. Rehnani and M. truncatula line A17 were grown on MS medium. After 4-6 weeks, segments of leaf and stem from two species were transferred to MS medium containing 2 mg L-1 NAA, 2,4-D and Kinetin. The results indicated that callus formation from leaf explants of M. sativa was higher than M. trancatula. In the next stage, media with different combinations of auxin, cytokinin or ethinyl estradiol were provided for regeneration. Then in two stages, explants of leaf and stem of two species were transferred on these media. Results after 3-6 weeks showed that in medium containing NAA and TDZ, stem pieces of M. sativa produced shoots while leaf pieces on NAA and ethinyl estradiol formed roots. Leaf explants of M. truncatula in the medium containing NAA and BAP, produced somatic embryos. Also in media with auxin and ethinyl estradiol, somatic embryos were formed on calli of two species. Ethinyl estradiol and auxin together can induce somatic embryogenesis and root production on calli and stem or leaf explants.
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How to cite this article:

F. Hoori, A.A. Ehsanpour and A. Mostajeran, 2007. Comparison of Somatic Embryogenesis in Medicago sativa and Medicago truncatula . Pakistan Journal of Biological Sciences, 10: 481-485.

DOI: 10.3923/pjbs.2007.481.485

URL: https://scialert.net/abstract/?doi=pjbs.2007.481.485

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