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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 24 | Page No.: 4527-4530
DOI: 10.3923/pjbs.2007.4527.4530
 
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Mutation of Streptomyces griseoflavus in Order to Obtain High Yield Desferrioxamine Producing Fused Cells

A. Akbarzadeh, D. Norouzian , A. Farhangi , M.R. Mehrabi , M. Shafiei , D. Zare , Z. Saffari , M. Mortazavi , M. Mardaneh and Z. Nemati

Abstract:
Streptomyces griseoflavus PTCC 1130 was mutated by UV irradiation. Two mutants were obtained (C7031 and S7011). These two mutants were able to produce desferioxamine. Desferrioxamine was extracted from the culture broth of the two mutated strains and the thin layer chromatogram of the products showed the RF values of 0.461, 0.463 and 0.456 for S7011, C7031 and the standard, respectively. The protoplasts of mutated Streptomyces griseoflavus were isolated and fused together. Total numbers of 58 fusions were obtained and only 17 fusions showed significant resistance to sodium azide and crystal violet. In terms of production of desferrioxamine only fusion PF9 and PF10 increased 68.3 and 81.8% desferrioxamine production as compared to parent strain (PTCC 1130), respectively.
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How to cite this article:

A. Akbarzadeh, D. Norouzian , A. Farhangi , M.R. Mehrabi , M. Shafiei , D. Zare , Z. Saffari , M. Mortazavi , M. Mardaneh and Z. Nemati , 2007. Mutation of Streptomyces griseoflavus in Order to Obtain High Yield Desferrioxamine Producing Fused Cells. Pakistan Journal of Biological Sciences, 10: 4527-4530.

DOI: 10.3923/pjbs.2007.4527.4530

URL: https://scialert.net/abstract/?doi=pjbs.2007.4527.4530

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