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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 22 | Page No.: 4118-4122
DOI: 10.3923/pjbs.2007.4118.4122
In vitro Plant Regeneration from Direct and Indirect Organogenesis of Memordica charantia
Saima Malik, Muhammad Zia, Riaz-ur-Rehman and M. Fayyaz Chaudhary

Abstract:
The study was conducted to analyze the effect of plant growth regulators on callogenesis and direct and indirect organogenesis of Memordica charantia. Callus cultures were induced from leaf, stem and cotyledonary explants of Momordica charantia, at different auxin and cytokinin concentrations either in single or in combination in MS medium. The best callogenic response was observed from all three explants (leaf, stem and cotyledon) on MS medium supplemented with 1.0 and 1.5 mg L‾1 BAP with 1.5 mg L‾1 NAA and 1.0 mg L‾1 2,4-D, respectively. The callus produced was hard, green and compact. These totipotent cells were failed to give rise shooting response when transferred to same or different growth regulator containing medium as second subculture. Indirect organogenesis response was very low or absent due to hardening of callus and habitutation. Best shooting was observed at 1.0 mg L‾1 BAP + 0.1 TDZ and 1.5 mg L‾1 BAP + 0.2 mg L‾1 NAA from shoot tip and cotyledonary node explants. While root formation was achieved when generated shoots were transferred to MS medium both full and half strength supplemented with different auxin concentrations.
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How to cite this article:

Saima Malik, Muhammad Zia, Riaz-ur-Rehman and M. Fayyaz Chaudhary, 2007. In vitro Plant Regeneration from Direct and Indirect Organogenesis of Memordica charantia. Pakistan Journal of Biological Sciences, 10: 4118-4122.

DOI: 10.3923/pjbs.2007.4118.4122

URL: https://scialert.net/abstract/?doi=pjbs.2007.4118.4122

 
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