
	Pakistan Journal of Biological Sciences
	Year: 2007 \| Volume: 10 \| Issue: 22 \| Page No.: 4075-4080 DOI: 10.3923/pjbs.2007.4075.4080

Restriction Fragment Length and Single Strand Conformational Polymorphisms in Chicken Mitochondrial Phosphoenol-Pyruate Carboxykinase Gene and its Association with Egg Production

Adam Torkamanzehi and Urs Kuhnlein

Abstract: This study analysed mitochondrial phosphoenol-pyruate carboxykinase (PEPCK-M) gene as a candidate QTL for egg production traits in chickens. Single Strand Conformational Polymorphism (SSCP) of a 300 bp DNA fragment, from exon 9 of samples from an egg laying North American commercial White Leghorn stock, revealed a total of 6 different single strand conformers, indicative of 3 alleles. Subsequent DNA sequencing found a total of 4 base changes in this fragment between these alleles (called A₁, A₂ and A₃) when compared to the reference sequence published online. The A₁ allele had one transition mutation of T to C at position 1700. The A₂ allele had accumulated three transition mutations: T to C at position 1578, A to G at position 1647 and T to C at position 1650. Transition mutation of T to C at position 1578 of the A₂ allele results in the loss of an AccI site, hence, producing a de novo RFLP. Analysis of 358 female individuals from this strain showed that the population is highly polymorphic at this site. The effect of PEPCK-M genotypes at this site, namely AccI -/-, AccI +/- and AccI +/+, was tested on three traits, age at first egg, egg production rate and egg number. Least square analysis showed that exon 9 RFLP significantly affects age at first egg (p<0.05). Egg production rate and egg number traits were not affected by different genotypes at this position. The data also indicates an over-dominance effect for the associated trait.

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How to cite this article:

Adam Torkamanzehi and Urs Kuhnlein, 2007. Restriction Fragment Length and Single Strand Conformational Polymorphisms in Chicken Mitochondrial Phosphoenol-Pyruate Carboxykinase Gene and its Association with Egg Production. Pakistan Journal of Biological Sciences, 10: 4075-4080.

DOI: 10.3923/pjbs.2007.4075.4080

URL: https://scialert.net/abstract/?doi=pjbs.2007.4075.4080

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