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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 22 | Page No.: 4015-4021
DOI: 10.3923/pjbs.2007.4015.4021
 
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Detection of Legionella pneumophila by PCR-ELISA Method in Industrial Cooling Tower Water

Soheili Majid, Nejadmoghaddam Mohammad Reza, Babashamsi Mohammad, Ghasemi Jamileh and Jeddi Tehrani Mahmood

Abstract:
Water supply and Cooling Tower Water (CTW) are among the most common sources of Legionella pneumophila (LP) contamination. A nonradio active method is described to detect LP in industrial CTW samples. DNA was purified and amplified by nested -PCR with amplimers specific for the 16s rRNA gene of LP. The 5 end biotinylated oligomer probe was immobilized on sterptavidin B coated microtiter plates. The nested-PCR product was labeled with digoxigenin and then hybridized with 5-biotinylated probes. The amplification products were detected by using proxidase-labled anti dioxygenin antibody in a colorimetric reaction. The assay detected LP present in 1 L of 5 CTW samples examined. All of the samples were Legionella positive in both culture and PCR-ELISA methods. The PCR-ELISA assay appears to exhibit high specificity and is a more rapid technique in comparison with bacterial culture method. Thus could prove suitable for use in the routine examination of industrial CTW contamination.
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How to cite this article:

Soheili Majid, Nejadmoghaddam Mohammad Reza, Babashamsi Mohammad, Ghasemi Jamileh and Jeddi Tehrani Mahmood, 2007. Detection of Legionella pneumophila by PCR-ELISA Method in Industrial Cooling Tower Water. Pakistan Journal of Biological Sciences, 10: 4015-4021.

DOI: 10.3923/pjbs.2007.4015.4021

URL: https://scialert.net/abstract/?doi=pjbs.2007.4015.4021

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