Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 21 | Page No.: 3931-3935
DOI: 10.3923/pjbs.2007.3931.3935
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Comparative Production of 6-Aminopenicillanic Acid by Different E. coli Strains and Their Acridine Orange (AO) Induced Mutants

Rubina Arshad , Shafqat Farooq and Syed Shahid Ali

Abstract:
The present study was conducted to see the difference in production of 6-APA I) between wild strains of E. coli collected from local environment and their acridine orange (AO) induced mutants and ii) between mutants and E. coli strains (ATCC 11105 and ATCC 9637) of American Type Culture Collection (ATCC) used commercially for enzymatic production of 6-APA. The optimum conditions for bioconversion were standardized and 6-APA was obtained in crystalline form. Relative PGA activity of local and foreign E. coli strains varied significantly with the highest being 12.7 in mutant strain (BDCS-N-M36) and the lowest 4.3 mg 6-APA h-1mg-1 wet cells in foreign strain (ATCC 11105). The enzyme activity exhibited by mutant strain (BDCS-N-M36) was also two folds higher compared to that in wild parent BDCS-N-W50 (6.3 mg 6-APA h-1 mg-1 wet cells). The overall production of 6-APA and conversion ratios ranged between 0.25-0.41 g of 6-APA per 0.5 g of penicillin G and 51-83%, respectively. Maximum conversion ratio (83%) was achieved by using crude cells of mutant strain (BDCS-N-M36) which is the highest value ever reported by crude cells on a shake-flask scale whereas reported 6-APA production by immobilized cells is 60-90% in batch and continuous systems. Results are being discussed with reference to importance of local bacterial strains and their significance for industrially important enzymes.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Rubina Arshad , Shafqat Farooq and Syed Shahid Ali , 2007. Comparative Production of 6-Aminopenicillanic Acid by Different E. coli Strains and Their Acridine Orange (AO) Induced Mutants. Pakistan Journal of Biological Sciences, 10: 3931-3935.

DOI: 10.3923/pjbs.2007.3931.3935

URL: https://scialert.net/abstract/?doi=pjbs.2007.3931.3935

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom