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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 21 | Page No.: 3880-3884
DOI: 10.3923/pjbs.2007.3880.3884
 
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Effect of Sodium Chloride on Establishment of Callus and Organogenesis in Brassica napus L.

F. Chamandoosti

Abstract:
In order to produce salt tolerant canola (Brassica napus L.) plants hypocotyl segments of its that were excised from 7 days old-seedlings cultured in MS medium with various concentrations of PGRs (NAA, IBA, 2, 4-D, KN and BA) and sodium chloride. The best media for induction and growth of callus (6 mg L-1 2, 4-D and 2 mg L-1 BA), production of adventitious shoots (1 mg L-1 IBA and 1 mg L-1 BA) and roots (1 mg L-1 NAA and 0.5 mg L-1 KN) were determined. Then explants cultured in these media plus 0-273.53 mM L-1 sodium chloride. Explants in these media established and in some of concentrations of sodium chloride (68.37-102.56 mM L-1) produced calli and adventitious shoots and roots better than the same media but free from sodium chloride. This finding suggests that somaclonal variation can yield stable-tolerant canola plants.
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How to cite this article:

F. Chamandoosti , 2007. Effect of Sodium Chloride on Establishment of Callus and Organogenesis in Brassica napus L.. Pakistan Journal of Biological Sciences, 10: 3880-3884.

DOI: 10.3923/pjbs.2007.3880.3884

URL: https://scialert.net/abstract/?doi=pjbs.2007.3880.3884

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