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Pakistan Journal of Biological Sciences

Year: 2007 | Volume: 10 | Issue: 21 | Page No.: 3880-3884
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Authors

F. Chamandoosti

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Research Article

Effect of Sodium Chloride on Establishment of Callus and Organogenesis in Brassica napus L.

F. Chamandoosti
In order to produce salt tolerant canola (Brassica napus L.) plants hypocotyl segments of its that were excised from 7 days old-seedlings cultured in MS medium with various concentrations of PGRs (NAA, IBA, 2, 4-D, KN and BA) and sodium chloride. The best media for induction and growth of callus (6 mg L-1 2, 4-D and 2 mg L-1 BA), production of adventitious shoots (1 mg L-1 IBA and 1 mg L-1 BA) and roots (1 mg L-1 NAA and 0.5 mg L-1 KN) were determined. Then explants cultured in these media plus 0-273.53 mM L-1 sodium chloride. Explants in these media established and in some of concentrations of sodium chloride (68.37-102.56 mM L-1) produced calli and adventitious shoots and roots better than the same media but free from sodium chloride. This finding suggests that somaclonal variation can yield stable-tolerant canola plants.
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