Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 17 | Page No.: 2955-2959
DOI: 10.3923/pjbs.2007.2955.2959
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Genetic Relationships Among Six Iranian Goat Populations Based on Random Amplified Polymorphic DNA Markers
Saeid Esmaeelkhanian, Ali Javanrouh Aliabad and Hamidreza Seyedabadi

Abstract:
The objectives of this study were to assess the genetic variability among six Iranian goat breeds including: Markhoz (MR), Korki of South Khorasan (KK), Black Lori (BL), Najdi (NJ), Korki of Raeini (KR), Tali (TL) and to evaluate the genetic relationships between populations using RAPD markers. The genetic characterization of these genetic resources is essential to conservation and breeding programs. Blood sample (50 individuals per breeds) were collected from spreading location of these breeds. DNA extraction was carried out by Salting-Out method. Initially, a total of 16 ten and eleven-nucleotide arbitrary primers were used but 10 of 16 primers revealed a pattern with scorable amplified bands. From a total number of 115 scored bands 62 (53.9%) and 53 (46.1%) were described as polymorphic and monomorphic, respectively. The average number of bands per primer 11/5 and with sizes varying from 220 to 2310 bp in length. Nei’s genetic distances varied between 0.081 and 0.227 in the populations. The phylogenetic tree was reconstructed on Neighbor-Joining method and showed two main separated groups. One includes KK, TL, KR in a branch and then NJ. Another consists BL and MR. This research was showed that RAPD technique is an useful tool for evaluation of genetic variation among of domesticated animals.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    The Polymorphisms of Goat THRSP Gene Associated with Ecological Factors in Chinese Indigenous Goat Breeds with Different Lipogenesis Ability
How to cite this article:

Saeid Esmaeelkhanian, Ali Javanrouh Aliabad and Hamidreza Seyedabadi, 2007. Genetic Relationships Among Six Iranian Goat Populations Based on Random Amplified Polymorphic DNA Markers. Pakistan Journal of Biological Sciences, 10: 2955-2959.

DOI: 10.3923/pjbs.2007.2955.2959

URL: https://scialert.net/abstract/?doi=pjbs.2007.2955.2959

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

       

       

Curve Bottom