Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 17 | Page No.: 2910-2914
DOI: 10.3923/pjbs.2007.2910.2914
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Study of Sugar Beet Cyst Nematode Life Cycle Using Plant Tissue Culture Method

Atena Shadmehr, Payman Nouroozi, Ghasemali Garousi and Ali-Reza Ahmadi

Abstract:
After optimization of sterilizing of cyst and larva second stage of Heterodera schachtii, possibility of using nematode on seedlings of sugar beet (Beta vulgaris L.) in in vitro conditions were studied using sterilized larvae of beet cyst nematode. For this purpose, non sterile cysts were extracted from infected soil and hatched into zinc chloride solution with concentration of 0.5 g L-1. Then, for preparation of sterile second stage larvae, several sterilizing treatments were used. Mean comparisons were performed between sterilized live larvae number by Duncan's method. Results showed that 70% ethanol for 1 min followed by 2.5% hypochlorite sodium for 5 minutes and 0.1% hypochlorite sodium for 20 min were best treatments for disinfecting cysts and larvae, respectively. In parallel, two nematode susceptible sugar beet varieties were applied to produce seedlings in in vitro culture. PGoB medium containing different hormonal compositions was used for producing of hairy roots and inoculation of seedling with sterilized larvae. After nematode inoculation tests, daily observations were done by counting cysts and stained roots and larvae under stereomicroscope. Between 5-12 cysts formed on the roots of each seedling from two varieties 40 days after inoculation. As a result, it seems that this technique can be used for sugar beet germplasm evaluation to screen nematode resistant genotypes in in vitro controlled condition.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Atena Shadmehr, Payman Nouroozi, Ghasemali Garousi and Ali-Reza Ahmadi, 2007. Study of Sugar Beet Cyst Nematode Life Cycle Using Plant Tissue Culture Method. Pakistan Journal of Biological Sciences, 10: 2910-2914.

DOI: 10.3923/pjbs.2007.2910.2914

URL: https://scialert.net/abstract/?doi=pjbs.2007.2910.2914

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom