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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 10 | Page No.: 1611-1617
DOI: 10.3923/pjbs.2007.1611.1617
 
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Isolation and Identification of Peanut Leaf Proteins Regulated by Water Stress

Chutipong Akkasaeng, Napaporn Tantisuwichwong, Issariya Chairam, Narumon Prakrongrak, Sanun Jogloy and Aran Pathanothai

Abstract:
Water deficits trigger signaling cascades leading to modulation of protein expression in plant tissues. Identification of peanut leaf proteins regulated by water stress provides some insights of cellular and molecular response of peanut plants to drought stress. Peanut variety Khon Kaen 4, a water-stress sensitive variety, was grown in a growth chamber under controlled environment. Water stress was imposed on day 30 after seedling emergence by withholding watering peanut plants for 6 days as compared to plants adequately supplied with water. Total protein were prepared from a leaflet of fully expanded leaf on the main stem. Proteins were separated in duplicated gels using two-dimensional gel electrophoresis and visualized by silver nitrate staining. Image analysis was performed using ImageMaster 2D Platinum 5.0 to determine proteins regulated by water stress. Molecular mass and isoelectric point of each regulated protein were used in database queries for protein identification. One protein was induced under water stress and the homologous protein was identified as Serine/threonine-protein phosphatase PP 1. Five proteins were down-regulated by water deficit. The homologous proteins were chaperone protein DNAJ, auxin-responsive protein IAA29, peroxidase 43, caffeoyl-CoA O-methyltransferase and SNF1-related protein kinase regulatory subunit beta-2. Down-regulated proteins may be associated with sensitivity of the peanut variety to water stress.
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How to cite this article:

Chutipong Akkasaeng, Napaporn Tantisuwichwong, Issariya Chairam, Narumon Prakrongrak, Sanun Jogloy and Aran Pathanothai, 2007. Isolation and Identification of Peanut Leaf Proteins Regulated by Water Stress. Pakistan Journal of Biological Sciences, 10: 1611-1617.

DOI: 10.3923/pjbs.2007.1611.1617

URL: https://scialert.net/abstract/?doi=pjbs.2007.1611.1617

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