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Pakistan Journal of Biological Sciences
  Year: 2007 | Volume: 10 | Issue: 1 | Page No.: 132-136
DOI: 10.3923/pjbs.2007.132.136
 
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Agrobacterium rhizogenes Mediated-transformation of Asimina triloba L. Cuttings

Tomas Ayala-Silva, Caula A. Beyl and Greg Dortch

Abstract:
The current research compared rooting of pawpaw, with softwood cuttings from mature stands, cuttings from the terminal 3-4 inches of seedlings and cuttings taken from shoots of rooted cuttings from seedlings. Four treatments were used: Agrobacterium rhizogenes strain MSU-1 (A4 wild type), A. rhizogenes strain MT232 (TR105 mutant), indole-3-butyric acid at 20,000 mg L-1 and control. Only the A. rhizogenes treatments induced rooting and only of the seedling cuttings. No statistical differences in rooting percentage were found among the bacterial strains. While the MSU-1 strain produced more roots, these were shorter and thinner than those produced by MT232 strain. No roots resulted from any other treatment. Roots obtained from infected seedlings were analyzed for transformation using polymerase chain reaction targeting the rolB and rolC vir genes. Roots confirmed as transgenic were 100% for both rolB and rolC.
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How to cite this article:

Tomas Ayala-Silva, Caula A. Beyl and Greg Dortch, 2007. Agrobacterium rhizogenes Mediated-transformation of Asimina triloba L. Cuttings. Pakistan Journal of Biological Sciences, 10: 132-136.

DOI: 10.3923/pjbs.2007.132.136

URL: https://scialert.net/abstract/?doi=pjbs.2007.132.136

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