
	Pakistan Journal of Biological Sciences
	Year: 2006 \| Volume: 9 \| Issue: 1 \| Page No.: 54-59 DOI: 10.3923/pjbs.2006.54.59

The Effects of Abscisic Acid and CaCl₂ on the Activities of Antioxidant Enzymes under Cold Stress in Maize Seedlings in the Dark

M. Khorshidi and A.M. Nojavan

Abstract: The aim of this study was to determine the effects of Abscisic acid, CaCl₂ and acclimation (15°C) pre-treatments on the activities of antioxidant enzyme system under cold stress (5°C) in maize seedlings in the dark. When corn seedlings were imposed to cold stress for 7 day at 5°C in the dark, antioxidant enzyme system was induced and protected the seedlings. At first, malondialdehyde (MDA) levels as a product of lipid peroxidation were measured. ABA at 0.1 ρM and CaCl₂ at 0.75 mMdecreased MDA levels in roots and shoots significantly, indicating that antioxidant enzymes are activated and cold injury is reduced. Measuring the relative activities of SOD, APX, CAT, GR and GPX revealed that, ABA and CaCl₂ promoted their activities in roots and shoots significantly. The enhancing effects of ABA on the activities of all enzymes under cold stress were higher than CaCl₂ and 15°C acclimation temperature. Calcium treatments were second in this respect. The role of ABA and Ca²⁺ was discussed in this study.

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How to cite this article:

M. Khorshidi and A.M. Nojavan, 2006. The Effects of Abscisic Acid and CaCl₂ on the Activities of Antioxidant Enzymes under Cold Stress in Maize Seedlings in the Dark. Pakistan Journal of Biological Sciences, 9: 54-59.

DOI: 10.3923/pjbs.2006.54.59

URL: https://scialert.net/abstract/?doi=pjbs.2006.54.59

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The Effects of Abscisic Acid and CaCl2 on the Activities of Antioxidant Enzymes under Cold Stress in Maize Seedlings in the Dark

The Effects of Abscisic Acid and CaCl₂ on the Activities of Antioxidant Enzymes under Cold Stress in Maize Seedlings in the Dark