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Pakistan Journal of Biological Sciences
  Year: 2006 | Volume: 9 | Issue: 2 | Page No.: 302-306
DOI: 10.3923/pjbs.2006.302.306
 
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In vitro Plant Regeneration from Callus of Cotyledons in Canola (Brassica napus L.)

F. Chamandosti, A. Majd and S. Mehrabian

Abstract:
In this study a simple and efficient procedure was developed for inducing callus and plant regeneration using hypocotyl segmsents of Canola (Brassica napus L.). The combinations and concentrations of differents plant growth regulators had a critical effect for callus formation with various colour and potential of differentiation. Three morphologically distinct types of calli were induced: 1) yellow calli were induced on MS medium supplemented with variou concentrations of 2, 4-D and 2 mg L-1 BA and presence of somatic embryos in first stages of development was showed by histological studies in these calli. white calli were induced on MS medium supplemented with into 1-2 mg L-1 IBA and 1 mg L-1 BA and MS medium with only 2 mg L-1 BA. These calli were organogen. So that differentiated to shoots, then shoots rooted and whole plants were obtained. And dark brown calli that induced on media with out of 2 mg L-1 IBA. These calli died and did not differentiated.
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How to cite this article:

F. Chamandosti, A. Majd and S. Mehrabian , 2006. In vitro Plant Regeneration from Callus of Cotyledons in Canola (Brassica napus L.). Pakistan Journal of Biological Sciences, 9: 302-306.

DOI: 10.3923/pjbs.2006.302.306

URL: https://scialert.net/abstract/?doi=pjbs.2006.302.306

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