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Pakistan Journal of Biological Sciences

Year: 2006 | Volume: 9 | Issue: 2 | Page No.: 302-306
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Authors

F. Chamandosti

A. Majd

S. Mehrabian

Keywords

Research Article

In vitro Plant Regeneration from Callus of Cotyledons in Canola (Brassica napus L.)

F. Chamandosti, A. Majd and S. Mehrabian
In this study a simple and efficient procedure was developed for inducing callus and plant regeneration using hypocotyl segmsents of Canola (Brassica napus L.). The combinations and concentrations of differents plant growth regulators had a critical effect for callus formation with various colour and potential of differentiation. Three morphologically distinct types of calli were induced: 1) yellow calli were induced on MS medium supplemented with variou concentrations of 2, 4-D and 2 mg L-1 BA and presence of somatic embryos in first stages of development was showed by histological studies in these calli. white calli were induced on MS medium supplemented with into 1-2 mg L-1 IBA and 1 mg L-1 BA and MS medium with only 2 mg L-1 BA. These calli were organogen. So that differentiated to shoots, then shoots rooted and whole plants were obtained. And dark brown calli that induced on media with out of 2 mg L-1 IBA. These calli died and did not differentiated.
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