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Pakistan Journal of Biological Sciences

Year: 2006 | Volume: 9 | Issue: 14 | Page No.: 2657-2661
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Authors

Nathanon Trachoo

Chantelle Boudreaux

Anuchita Moongngarm

Supachai Samappito

Rumpai Gaensakoo

Keywords

Effect of germinated rice media on growth of probiotic bacteria including Lactobacillus acidophilus, L. pentosus, L. plantarum and L. fermentum were studied. Germinated rough rice powder was obtained by allowing rice grains to germinate in water with hulls at 80% relative humidity and 30°C for 48 h. The germinated rice grains were dried, dehulled and powdered with a laboratory hamper mill. The powder (5% w/w) was well-mixed with water to prepare the growth medium for the probiotic bacteria strains. This was compared with medium with regular rice powder. NaCl (2%w/w) was also added to study the bacteria growth. Chemical analysis including vitamins B (thiamin, niacin and pyridoxine), reducing sugar and total protein contents in rice, brown rice and germinated rough rice were determined. Germination of rice grains increased many nutrients. Vitamins B, reducing sugar and total protein contents of germinated rough rice were higher (p<0.05) than those of brown rice and rice, respectively. Addition of NaCl lowered the growth of probiotic bacteria. L. plantarum exhibited the highest (p<0.05) growth in medium containing germinated rough rice powder with and without NaCl compared to the other strains. The results from this present study suggested that germinated rough rice can be used as a supplement for L. plantarum in food fermentation.
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