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Pakistan Journal of Biological Sciences
  Year: 2006 | Volume: 9 | Issue: 14 | Page No.: 2586-2592
DOI: 10.3923/pjbs.2006.2586.2592
 
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In vitro Study Involving the Comparative Effect of Heavy Metal Ions on Antioxidant Enzymes Activity and Lipid Peroxide Levels in Human Erythrocytes

Ahmed Hussein Al-Mustafa

Abstract:
The effect of the concentration rise in Cu, Zn, Pb and Cr satls (10-100 μM) on lipid peroxidation (MDA) and the activities of peroxide metabolism enzymes: catalase, glutathione peroxidase, reductase and glutathione s-transferases were investigated in human erythrocytes. Copper and lead were found to have more pronounced effect than zinc and chrome on the erythrocytes cell membrane phospholipid oxidation and MDA formation. In human erythrocytes, Cr significantly increased the glutathione peroxidase (GPx) and decreased the Glutathione Reductase (GR), while no significant difference (p<0.05) in glutathione s-transferase (GST) and catalase (CAT) activities at 100 μM were observed. Zinc increased the GPx and CAT and decreased the GR, whereas GST activities do not changed (p<0.05). Lead significantly increased GPx and GST and no effect on GR and CAT activities was observed (p<0.05). However, copper showed no significant effect on GPx and GST activities, but significantly caused increased in CAT and a decrease in GR activity (p<0.001). These data show that, heavy metal ions had only little effect on haemolysis and statistical variable effects on antioxidant enzyme activities in human erythrocytes and lipid peroxide level when compared to control values. It was concluded that metal ions produced variable toxic effects on human erythrocytes, so that the mechanism Cu and Pb-induced membrane lipid oxidation may be different from that caused by Zn and Cr ions.
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How to cite this article:

Ahmed Hussein Al-Mustafa , 2006. In vitro Study Involving the Comparative Effect of Heavy Metal Ions on Antioxidant Enzymes Activity and Lipid Peroxide Levels in Human Erythrocytes. Pakistan Journal of Biological Sciences, 9: 2586-2592.

DOI: 10.3923/pjbs.2006.2586.2592

URL: https://scialert.net/abstract/?doi=pjbs.2006.2586.2592

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