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Pakistan Journal of Biological Sciences
  Year: 2006 | Volume: 9 | Issue: 14 | Page No.: 2579-2585
DOI: 10.3923/pjbs.2006.2579.2585
 
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High Temperature Responses of Aegilops biuncialis Species and Triticum durum Cultivar

Serpil Terzioglu, Mustafa Yildiz, M. Yuceland and H. Avni Oktem

Abstract:
Thermal tolerances and heat shock protein (s) [HSP(s)] of wild wheat species (Aegilops biuncialis Vis.) and Triticum durum Desf. cv. Harran-95 were investigated in etiolated seedlings. The thermal tolerances of the genotypes were determined by growth experiment and cell viability test. The HSPs were separated by two-dimensional (2-D) gel electrophoresis technique. In all the experiments, the two-day old etiolated seedlings were exposed to control (23°C, 24 h), acclimation (37°C, 24 h) and heat stress (37°C, 24 h¬50°C, 1 h) temperature treatments. In growth experiment, there was no significant difference between the values of acquired thermal tolerance (%) of Ae. biuncialis and cv. Harran-95 in all treatments. In cell viability test, the values of acquired thermal tolerance (%) for Ae. biuncialis and cv. Harran-95 were detected as 54.5 and 28.4%, respectively. The molecular weights of HSPs synthesized in coleoptile tissues ranged from 19.7 kDa to 67.5 kDa and pI values ranged from 6.3 to 7.3. Most of the HSPs synthesized at acclimation treatment disappeared at heat stress treatment in Ae. biuncialis. Four newly synthesized HSPs with molecular weights of 19.7, 19.7, 25.9 and 26.6 kDa (pI values of 6.6, 6.5, 6.6 and 6.4, respectively) were common between Ae. biuncialis and cv. Harran-95 at both acclimation and heat stress treatments compared to control.
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How to cite this article:

Serpil Terzioglu, Mustafa Yildiz, M. Yuceland and H. Avni Oktem, 2006. High Temperature Responses of Aegilops biuncialis Species and Triticum durum Cultivar. Pakistan Journal of Biological Sciences, 9: 2579-2585.

DOI: 10.3923/pjbs.2006.2579.2585

URL: https://scialert.net/abstract/?doi=pjbs.2006.2579.2585

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