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Pakistan Journal of Biological Sciences
  Year: 2006 | Volume: 9 | Issue: 2 | Page No.: 227-230
DOI: 10.3923/pjbs.2006.227.230
 
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Detection of Calicivirus Genome in Calves Using Ni/E3 Primers in Shahrekord Area, Iran

M. Mahzounieh, E. Moghtadaei and T. Zahraei Salehi

Abstract:
Caliciviruses may cause a wide spectrum of disease in animals and are important etiological agent of viral gastroenteritis in humans. Members of the family caliciviridae are small nonenveloped viruses 27 to 35 nm in diameter. They possess a single stranded poly adenylated RNA genome. Caliciviruses have been isolated from mink, dog, cattle and non-human primates. Norwalk-like Viruses (NLVs) are the most common cause of acute non-bacterial gastroenteritis in humans. Cattle may be a reservoir of NLVs although never bovine NLVs have been found in humans. In this study, we try to detect enteric caliciviruses genome from 50 fecal samples of 6 dairy cattle herds in Shahrekord area using reverse transcriptase polymerase chain reaction (RT-PCR) assays specific for NLVs found in humans. The primers used for PCR amplification were Ni and E3, which amplify a 113 bp product for the detection of both genogroups I and II SRSV RNA in fecal material. Our results showed that nine specimens (18%) were positive. These findings suggest that calicivirus infection is endemic in dairy herds in Shahrekord, Iran and may be have an important role in calf diarrhoea.
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How to cite this article:

M. Mahzounieh, E. Moghtadaei and T. Zahraei Salehi, 2006. Detection of Calicivirus Genome in Calves Using Ni/E3 Primers in Shahrekord Area, Iran. Pakistan Journal of Biological Sciences, 9: 227-230.

DOI: 10.3923/pjbs.2006.227.230

URL: https://scialert.net/abstract/?doi=pjbs.2006.227.230

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