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Pakistan Journal of Biological Sciences
  Year: 2006 | Volume: 9 | Issue: 2 | Page No.: 201-204
DOI: 10.3923/pjbs.2006.201.204
 
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The Effect of GnRH Agonist (Buserelin) Treatment of Awasi Ewes on Day 12 Post-mating on Plasma Oestradiol Concentrations

Ghazi F. Basiouni

Abstract:
The main objective of this study was to examine the effect of treatment of Awasi ewes with GnRH agonist (buserelin) on day 12 post-summer induced mating on oestradiol concentrations. Ewes were divided into two groups (15 ewes/group). All ewes were synchronized for estrus using intravaginal progestagen sponges removed on day 12. Soon after sponges removal, all ewes were given an intramuscular 500 IU injection of PMSG. Natural mating was made by the introduction of two adult fertile rams (fited with raddles) to each group one day after PMSG injections. Twelve days after the start of mating, only ewes marked by the rams were injected intramuscularly with 10 micrograms of buserelin (12 ewes-treated), or injected with normal saline (10 ewes-control). Blood samples were taken from these ewes for the measurement of oestradiol concentrations at -36, -24, -12 h and immediately before the start of treatment at 0 hour and at 12, 24 and 36 h post-treatment. No significant differences were found in oestradiol concentrations before and after treatment with buserelin (1.78±0.10 and 1.66±0.11 pg mL-1, respectively). However, post-treatment oestradiol concentrations were found to be significantly higher (p<0.05) in the control group compared to pre-treatment values (2.07±0.15 and 1.78±0.10 pg mL-1, respectively). Furthermore, post-treatment oestradiol concentrations were found to be significantly higher (p = 0.025) in the control compared to buserelin treated ewes (2.07±0.15 and 1.66±0.11 pg mL-1, respectively). These results indicate that treatment of Awasi ewes with buserelin 12 days post-summer induced mating suppressed the increase in plasma oestradiol concentrations.
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How to cite this article:

Ghazi F. Basiouni , 2006. The Effect of GnRH Agonist (Buserelin) Treatment of Awasi Ewes on Day 12 Post-mating on Plasma Oestradiol Concentrations. Pakistan Journal of Biological Sciences, 9: 201-204.

DOI: 10.3923/pjbs.2006.201.204

URL: https://scialert.net/abstract/?doi=pjbs.2006.201.204

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