Research Article

**Formation of Catechin in Callus Cultures and Micropropagation of Rheum ribes L.**

The aim of this project was to study of the effects of different nutritional factors on the production of catechin in Rheum ribes L. and micropropagation of this species in agar-solidified medium. Different parts of Rheum ribes sterile seedlings were cultured on MS medium that supplemented with IBA (1 mg L^-1) and BA (1 mg L^-1) for generation of callus. The concentration of sucrose, vitamins and myo-inositol and ratio of NO₃^- to NH₄⁺ in the medium was changed and content of catechin was determined by HPLC.Myo-inositol 100 mg L^-1 in the medium that supplemented with 0.5 mg L^-1 IBA and 0.5 mg L^-1 BAP. Rooting medium was ½ MS salts + 0.5 mg L^-1 IBA. Regenerated plants were transferred to clay pots a mixture of soil, vermiculite and sand and maintained under 50-70% plants for the first 7 days.

PDF References Citation

How to cite this article

M. Farzami Sepehr and M. Ghorbanli, 2005. Formation of Catechin in Callus Cultures and Micropropagation of Rheum ribes L. . Pakistan Journal of Biological Sciences, 8: 1346-1350.

DOI: 10.3923/pjbs.2005.1346.1350

URL: https://scialert.net/abstract/?doi=pjbs.2005.1346.1350

Pakistan Journal of Biological Sciences

Article Trend

Total views 195

Search

Authors

M. Farzami Sepehr

M. Ghorbanli

Keywords

Research Article

**Formation of Catechin in Callus Cultures and Micropropagation of Rheum ribes L.**

How to cite this article

Leave a Comment