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Pakistan Journal of Biological Sciences
  Year: 2005 | Volume: 8 | Issue: 8 | Page No.: 1090-1095
DOI: 10.3923/pjbs.2005.1090.1095
Evaluation of the Antimicrobial Activity of Extract from Buntan (Citrus grandis Osbeck) Fruit Peel
Matook Saif Mokbel and Fumio Hashinaga

Abstract:
Antimicrobial substances were extracted from buntan (Citrus grandis Osbeck) fruit peel (flavedo and albedo) using various solvent such as n-hexane, ethyl acetate (EtOAc), butanol and methanol. The antimicrobial activity of the extracts was evaluated using the disc diffusion method, growth inhibition (%) with fungi growth and Minimum Inhibitory Concentration (MIC). Antimicrobial substance of EtOAc extracts of flavedo and albedo were fractionated using silica gel column chromatography (CC). Further purification to the albedo active fraction resulted in two active compounds, β-sitosterol (1) and oleic acid (2). Antibacterial activities of the compounds against Gram-negative and positive bacteria by using MIC and was 270-350 ppm in β-sitosterol and 150-350 ppm in oleic acid. Their extracts could well be useful to prevent oxidation in fruit juices and essential oils of food products as well as for health supplements.
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How to cite this article:

Matook Saif Mokbel and Fumio Hashinaga , 2005. Evaluation of the Antimicrobial Activity of Extract from Buntan (Citrus grandis Osbeck) Fruit Peel. Pakistan Journal of Biological Sciences, 8: 1090-1095.

DOI: 10.3923/pjbs.2005.1090.1095

URL: https://scialert.net/abstract/?doi=pjbs.2005.1090.1095

 
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