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Pakistan Journal of Biological Sciences
  Year: 2004 | Volume: 7 | Issue: 3 | Page No.: 331-334
DOI: 10.3923/pjbs.2004.331.334
Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Anther Culture of Rice
Md. Monirul Islam, Sanjay Kumar Adhikary , Purnendu Gain , Md. Mizanur Rahman and Noor-e-Alam Siddique

Abstract:
An experiment was conducted to find the effects of different concentrations and combinations of growth regulators viz. 2, 4 D, IAA, α-NAA and Kinetin on callus induction from the anthers of a commercial hybrid rice line IR-69690, developed by Bangladesh Rice Research Institute and subsequent plant regeneration. N6 medium was used as basal medium. Callus induction frequencies in different media combinations ranged from 1.2 to 35.5%. The medium supplemented with 2, 4 D 1 mg L-1, α-NAA 2 mg L-1 and Kinetin 1 mg L-1 was found most effective for callus induction (35.5%). Regeneration of plants from the callus on agarified MS medium supplemented with α-NAA 0.5 mg L-1 and Kinetin 3 mg L-1 was also variable and ranged from 16.7 to 69.3%. Calli derived from the media supplemented with 2, 4 D 1 mg L-1, α-NAA 2 mg L-1 and Kinetin 1 mg L-1 also showed better performance for plant regeneration (69.3%) and among these plants 56.14% were green. However the callus induction medium containing α-NAA 1 mg L-1 and Kinetin 1 mgL-1 directly produced green regenerated plants in higher frequency (70%), without transferring the calli on to the regeneration medium; but rate of callus induction in this medium was very low (6%).
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How to cite this article:

Md. Monirul Islam, Sanjay Kumar Adhikary , Purnendu Gain , Md. Mizanur Rahman and Noor-e-Alam Siddique , 2004. Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Anther Culture of Rice. Pakistan Journal of Biological Sciences, 7: 331-334.

DOI: 10.3923/pjbs.2004.331.334

URL: https://scialert.net/abstract/?doi=pjbs.2004.331.334

 
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