Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2004 | Volume: 7 | Issue: 10 | Page No.: 1706-1710
DOI: 10.3923/pjbs.2004.1706.1710
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Preliminary Investigation on the Antinociceptive Properties of Haruan (Channa striatus) Fillet Extracted with Various Solvent Systems

Z.A. Zakaria , M.N. Somchit , M.R. Sulaiman and A.M. Mat Jais

Abstract:
The present study was conducted with an aim of establishing the best organic solvent system that can yielded the Channa striatus extract with highest antinociceptive activity and also to determine the polarity of the respective bioactive compound using the acetic acid- induced abdominal constriction test. Briefly, the Channa striatus fillet were extracted separately with methanol, ethanol, chloroform, chloroform:methanol and distilled water overnight. This procedure is repeated three times and each of the supernatant obtained was pooled together and then subjected to the evaporation process. The extracts obtained was prepared in the doses of 0.5, 1.0, 2.0, 4.0 and 8.0 g kg-1 and administered subcutaneously (SC) into mice. Thirty min after the administration of extracts, 0.6% acetic acid was administered intraperitoneally (IP). All solutions were administered in the volume of 10 mL kg-1. From the data obtained, it can be concluded that the bioactive compounds to be of two types, the polar compound that is water-soluble and the non-polar compound that is fat-soluble. These two compounds might act alone, as in DH2O and chloroform extracts or act together, as in CM, methanol and ethanol extracts. Furthermore, the ability of this compound(s) to exhibit the antinociceptive activity in all the three types of solvents also suggested the presence of peptide compound. However, further investigation need to be carried out in order to isolate and identify the respective compound. In addition, methanol and chloroform:methanol were found to be the best solvents for further isolation of the respective bioactive compound of Channa striatus.
PDF References Citation Report Citation
 RELATED ARTICLES:
  •    Genetic Variation of Striped Snakehead Fish (Channa striata) in River Basin of Central Thailand Inferred from mtDNA COI Gene Sequences Analysis
How to cite this article:

Z.A. Zakaria , M.N. Somchit , M.R. Sulaiman and A.M. Mat Jais , 2004. Preliminary Investigation on the Antinociceptive Properties of Haruan (Channa striatus) Fillet Extracted with Various Solvent Systems. Pakistan Journal of Biological Sciences, 7: 1706-1710.

DOI: 10.3923/pjbs.2004.1706.1710

URL: https://scialert.net/abstract/?doi=pjbs.2004.1706.1710

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom