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Pakistan Journal of Biological Sciences

Year: 2003 | Volume: 6 | Issue: 2 | Page No.: 157-163
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Authors

N.Y. Chaudhry

Qurat-ul-Ain

Keywords

Research Article

Effect of Growth Hormones i.e., IAA, Kinetin and Heavy Metal i.e., Lead Nitrate on the Internal Morphology of Leaf of Phaseolus vulgaris L.

N.Y. Chaudhry and Qurat-ul-Ain
Histomorphology of the leaves of Phaseolus vulgaris L. were studied following treatments with growth hormones i.e., IAA and Kinetin and heavy metal i.e., Pb (NO3)2. The data was collected after 15 days and compared with control. In 50 ppm IAA, 50 ppm Kinetin as well as 50 ppm IAA+50 ppm Kinetin 3 additional leaf primordia were observed when compared with control while in all other treatments five leaves were observed. Moreover, application of 50 ppm IAA and 50 ppm Kinetin also registered an increase in leaf area, consequently the fresh and dry weight of leaves were also increased. While application of 50 ppm Pb, 50 ppm IAA+50 ppm Pb and 50 ppm Kinetin+50 ppm Pb revealed decrease in the above parameter. SLA also showed increase. The growth hormones also registered increase in the number of stomata, size of guard cells, as well as stomatal pore, stomatal index and %age of open stomata. Whereas, 50 ppm Pb and 50 ppm Pb+50 ppm Kinetin showed remarkable decrease in the %age of open stomata. Moreover, the number and size of palisade cells, width of spongy cells, width of abaxial and adaxial epidermal cells, number of xylem elements and width of metaxylem elements also showed increase when treated with 50 ppm IAA, 50 ppm Kinetin individually as well as in combinations. While all the above parameters showed decrease with the application of 50 ppm Pb alone and in combination with 50 ppm IAA and 50 ppm Kinetin.
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