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Characterization of Some Indigenous Mercury Resistant Bacteria from Polluted Environment |
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Shazia Afrasayab,
Azra Yasmin and Shahida Hasnain |
Abstract:
Among six Hg-resistant isolates (SHg-7, SHg-8, SHg-9, SHg-10, SHg-11, SHg-12), SHg-8 and SHg-12 could tolerate 450 μg ml–1 of HgCl2 in L-agar medium, whereas rest of the strains could tolerate 500 μg ml–1 of HgCl2 in this medium were Gram-negative rods (SHg-7, SHg-8, SHg-9, SHg-10, SHg-11) and SHg-12 were Gram-positive rods. SHg-11 & SHg-12 were strictly aerobic, remaining were facultatively aerobic. On the basis of morphological and biochemical characterization isolates SHg-7, SHg-8, SHg-9, SHg-10 could be affiliated with family Vibrionaceae and SHg-11 shared characters with Pseudomonadaceae, while the affinities of SHg-12 remained uncertain. All of them had a single plasmid band except SHg-11, but these plasmids were non-conjugative. They had broad pH range (5-11) with pH optima either at 6 or 7. Their optimal growth temperature was 32°C (SHg-9, SHg-10, SHg-11) and 37°C (SHg-7, SHg-8, SHg-12). All of them were sensitive to Sm, Km and Tc, while they showed resistance against Ap and Cm. They could also tolerate other heavy metal salts.
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How to cite this article:
Shazia Afrasayab, Azra Yasmin and Shahida Hasnain, 2002. Characterization of Some Indigenous Mercury Resistant Bacteria from Polluted Environment. Pakistan Journal of Biological Sciences, 5: 792-797.
DOI: 10.3923/pjbs.2002.792.797
URL: https://scialert.net/abstract/?doi=pjbs.2002.792.797
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