Research Article

PCR Amplification and Cloning of T-DNA Homologous Sequences from Nicotiana glauca

M. Qayyum Khan, Mukhtar Alam, R.J.K. Narayan and M. Ilyas Khan

Twenty six-mer forward and 23-mer reverse primers were designed using the published nucleotide sequence data of cellular T-DNA of Nicotiana glauca. Primers annealing temperature and Mg⁺²concentration were optimized for a polymerase chain reaction. At target sequence of about 1050 bp overlapping the rolB and rolC genes were successfully amplified from the genomic DNA of N. glauca. The amplified sequence was cloned into pUC18 vector. The cloned rol B-C fragment showed a high base sequence homology to the rol B and C genes located on the T-DNA of Ti and Ri plasmids of Agrobacterium tumefaciens and Agrobacterium rhizogenes respectively.

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M. Qayyum Khan, Mukhtar Alam, R.J.K. Narayan and M. Ilyas Khan, 2002. PCR Amplification and Cloning of T-DNA Homologous Sequences from Nicotiana glauca. Pakistan Journal of Biological Sciences, 5: 780-783.

DOI: 10.3923/pjbs.2002.780.783

URL: https://scialert.net/abstract/?doi=pjbs.2002.780.783

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Authors

M. Qayyum Khan

Mukhtar Alam

R.J.K. Narayan

M. Ilyas Khan

Keywords

Research Article

PCR Amplification and Cloning of T-DNA Homologous Sequences from Nicotiana glauca

How to cite this article

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