Research Article

Plasmid-Encoded Transferable Antibiotic Resistance in Gram-negative Bacteria, Isolated from Drinking Water in Ismailia City

A.M. Diab, M.H. Abdel Aziz and S.A . Selim

Thirteen multiple antibiotic resistant (MAR) gram-negative bacterial isolates from drinking water of Ismailia city were examined for the presence of plasmid DNA. Only six isolates were found to be plasmid-bearing isolates. One of these isolates, Aeromonas hydrophila, found to contain three plasmids with molecular sizes of 21.226, 3.530 and 1.375Kbp. Other isolates belonged to the genera Escherichia, Enterobacter (2 isolates), Pseudomonas and Salmonella which were found to contain only one plasmid each with varying molecular sizes. Curing and transformation protocols indicated that the ampicillin resistance of these isolates is plasmid-linked. Transformation coefficient, resistance expression within E. coli HB 101 and DH1 as well as restriction analysis of the plasmids were achieved.

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A.M. Diab, M.H. Abdel Aziz and S.A . Selim, 2002. Plasmid-Encoded Transferable Antibiotic Resistance in Gram-negative Bacteria, Isolated from Drinking Water in Ismailia City. Pakistan Journal of Biological Sciences, 5: 774-779.

DOI: 10.3923/pjbs.2002.774.779

URL: https://scialert.net/abstract/?doi=pjbs.2002.774.779

Pakistan Journal of Biological Sciences

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Authors

A.M. Diab

M.H. Abdel Aziz

S.A . Selim

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Research Article

Plasmid-Encoded Transferable Antibiotic Resistance in Gram-negative Bacteria, Isolated from Drinking Water in Ismailia City

How to cite this article

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