Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2002 | Volume: 5 | Issue: 7 | Page No.: 770-773
DOI: 10.3923/pjbs.2002.770.773
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

ALA-based Photodynamic Therapy of Jurkat Cells: Temperature Dependence

M. Idrish Miah

Abstract:
A study has been performed on the temperature dependence of the light exposure of human leukemic cells when treated with the photosensitizing agent δ-aminolevulinic acid, (ALA). The cells were incubated with 1mM ALA for 4h at different temperatures (31-45°C) and irradiated with different light doses in the blue spectral region (430nm). The dose-response curves were studied. Shoulders at the lower exposure regions were observed in the log-survival curves for incubation temperatures 31-37°C. The threshold dose values for the temperatures for which the shoulders had appeared were found to be 3.9, 3.2, 2.2, and 1.6min. for 31, 33, 35 and 37°C incubation temperatures, respectively. The phototoxic effect for the cells with these critical values was small. The activation energy for overcoming the shoulder reactions was 25kcal/mol. An activation energy of 9kcal/mol calculated for the cell type might be for the uptake of ALA by cells or protoporphyrin IX (PPIX) production in cells.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Toxicity Prediction of Photosensitizers Bearing Carboxylic Acid Groups by ECOSAR and Toxtree
  •    Spectroscopic Study of Porphyrin Production and Accumulation in Culture of Propionibacterium acnes
How to cite this article:

M. Idrish Miah , 2002. ALA-based Photodynamic Therapy of Jurkat Cells: Temperature Dependence. Pakistan Journal of Biological Sciences, 5: 770-773.

DOI: 10.3923/pjbs.2002.770.773

URL: https://scialert.net/abstract/?doi=pjbs.2002.770.773

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom