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Pakistan Journal of Biological Sciences
  Year: 2002 | Volume: 5 | Issue: 1 | Page No.: 75-79
DOI: 10.3923/pjbs.2002.75.79
Micropropagation and Plant Regeneration of Rauvolfia serpentina by Tissue Culture Technique
Shahrear Ahmad, M. N. Amin, M.A.K. Azad and M. Ashik Mosaddik

Abstract:
The shoots of Rauvolfia serpentina from the field growing plants were used as explants and cultured on MS medium supplemented with 2.5 mg/L BA + 0.1 mg/L NAA to afford multiple shoots. It was evident that MS + 0.5 mg/L BA + 0.2 mg/L NAA was the most suitable combination for induction of callus and development of shoot buds formation but it failed to support elongation of shoot. So, the cultures with tiny shoots was transferred to 2.0 mg/L BA with 0.05mg/L NAA, where they showed excellent elongation. Regenerated shoots rooted well 0.2 mg/L IBA+ 0.2mg/L NAA combination. After having acclimatization the regenerated plantlets were transferred to the open field where 95% of the plant-lets survived and showed excellent growth.
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How to cite this article:

Shahrear Ahmad, M. N. Amin, M.A.K. Azad and M. Ashik Mosaddik, 2002. Micropropagation and Plant Regeneration of Rauvolfia serpentina by Tissue Culture Technique. Pakistan Journal of Biological Sciences, 5: 75-79.

DOI: 10.3923/pjbs.2002.75.79

URL: https://scialert.net/abstract/?doi=pjbs.2002.75.79

 
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