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Pakistan Journal of Biological Sciences
  Year: 2002 | Volume: 5 | Issue: 6 | Page No.: 693-695
DOI: 10.3923/pjbs.2002.693.695
 
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Effects of Various Growth Regulators on Callus Formation and Regeneration in Brassica napus Cv. Oscar
Muhammad Ramzan Khan, Hamid Rashid and Azra Quraishi

Abstract:
Hypocotyl explants from in vitro seedlings were cultured aseptically on Gamborg (B5) medium supplemented with various concentrations and combinations of dichlorophenoxyacetic acid, indoleacetic acid, naphthalene acetic acid and benzylaminopurine for callus formation and shoot regeneration. 2,4-D alone @ 2 mg/l gave the highest callus formation (96%). The results revealed that BAP @ 2 mg/l with IAA 0.5 mg/l was the most appropriate combination to produce the multiple shoots (14.5 shoots per culture). Efficient rooting was achieved on half-strength MS medium supplemented with 0.250mg/I IBA and 0.125mg/l IAA. In vitro raised plantlets were transferred to potted soil and finally to experimental fields to assess their acclimatizing potential.
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How to cite this article:

Muhammad Ramzan Khan, Hamid Rashid and Azra Quraishi , 2002. Effects of Various Growth Regulators on Callus Formation and Regeneration in Brassica napus Cv. Oscar. Pakistan Journal of Biological Sciences, 5: 693-695.

DOI: 10.3923/pjbs.2002.693.695

URL: https://scialert.net/abstract/?doi=pjbs.2002.693.695

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