Effects of Various Growth Regulators on Callus Formation and Regeneration in Brassica napus Cv. Oscar


	Pakistan Journal of Biological Sciences
	Year: 2002 \| Volume: 5 \| Issue: 6 \| Page No.: 693-695 DOI: 10.3923/pjbs.2002.693.695

Effects of Various Growth Regulators on Callus Formation and Regeneration in Brassica napus Cv. Oscar

Muhammad Ramzan Khan, Hamid Rashid and Azra Quraishi

Abstract: Hypocotyl explants from in vitro seedlings were cultured aseptically on Gamborg (B₅) medium supplemented with various concentrations and combinations of dichlorophenoxyacetic acid, indoleacetic acid, naphthalene acetic acid and benzylaminopurine for callus formation and shoot regeneration. 2,4-D alone @ 2 mg/l gave the highest callus formation (96%). The results revealed that BAP @ 2 mg/l with IAA 0.5 mg/l was the most appropriate combination to produce the multiple shoots (14.5 shoots per culture). Efficient rooting was achieved on half-strength MS medium supplemented with 0.250mg/I IBA and 0.125mg/l IAA. In vitro raised plantlets were transferred to potted soil and finally to experimental fields to assess their acclimatizing potential.

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How to cite this article:

Muhammad Ramzan Khan, Hamid Rashid and Azra Quraishi , 2002. Effects of Various Growth Regulators on Callus Formation and Regeneration in Brassica napus Cv. Oscar. Pakistan Journal of Biological Sciences, 5: 693-695.

DOI: 10.3923/pjbs.2002.693.695

URL: https://scialert.net/abstract/?doi=pjbs.2002.693.695

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