Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 2002 | Volume: 5 | Issue: 10 | Page No.: 1070-1073
DOI: 10.3923/pjbs.2002.1070.1073
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Investigation of a PCR-based Method for Insecticide Resistance Monitoring

Riaz Shah, Karen Armstrong, Sue P. Worner and R. Bruce Chapman

Abstract:
The use of RAPD-PCR was investigated for its suitability in pesticide resistance monitoring. A high salt and Chelex 100 (BioRad) resin methods were used to extract DNA from susceptible and propargite-resistant strains of Tetranychus urticae Koch. The high salt method of DNA extraction was found suitable for extracting DNA from individual adult females of T. urticae. The DNA products obtained with the primer B8 were reproducible but inconsistant causing heterogeneity of PCR banding patterns. The inconsistant banding patterns could be due to the unknown genotypes of the females used in the analyses as it could not be confirmed that the susceptible and propargite-resistant strains of T. urticae were of SS (homozygous susceptible) and RR (homozygous resistant) genotypes. A second reason for the inconsistency of RAPD bands in this study could be the presence of non-mite DNA in the samples. These problems, when resolved, could make RAPD-PCR a potential monitoring tool for pesticide resistance detection and subsequent management.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Insecticides Resistance Detection in Field-collected Populations of Pectinophora gossypiella (Saunders)
How to cite this article:

Riaz Shah, Karen Armstrong, Sue P. Worner and R. Bruce Chapman , 2002. Investigation of a PCR-based Method for Insecticide Resistance Monitoring. Pakistan Journal of Biological Sciences, 5: 1070-1073.

DOI: 10.3923/pjbs.2002.1070.1073

URL: https://scialert.net/abstract/?doi=pjbs.2002.1070.1073

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom