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Pakistan Journal of Biological Sciences

Year: 2001 | Volume: 4 | Issue: 3 | Page No.: 266-270
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Authors

D. C. Ang

S. Abd-Aziz

H. M. Yusof

M. I. A. Karim

A. Ariff

K. Uchiyama

S. Shioya

Keywords

Research Article

Partial Purification and Characterisation of Amylolytic Enzymes Obtained from Direct Fermentation of Sago Starch to Ethanol by Recombinant Yeast

D. C. Ang, S. Abd-Aziz, H. M. Yusof, M. I. A. Karim, A. Ariff, K. Uchiyama and S. Shioya
Rice α-amylase and glucoamylase produced by S. cerevisiae YKU 107 and YKU 131, respectively were partial purified using anion exchange chromatography and characterised. The optimum active conditions for -amylase was at pH 5.0 and temperature of 50°C. It was capable in hydrolysing more than 85% of potato, rice, cassava and sago starch, however, it could only hydrolysed 64% of corn starch. Glucoamylase was optimally active at pH 5.5 and temperature of 55°C. Glucoamylase could hydrolyse more than 70% of cassava, sago and potato starch, it also hydrolysed more than 65% of corn and rice starch. Both of the enzymes were not severely affected by the changes of ionic strength of acetate buffer at molarity lower than 0.20 M.
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How to cite this article

D. C. Ang, S. Abd-Aziz, H. M. Yusof, M. I. A. Karim, A. Ariff, K. Uchiyama and S. Shioya, 2001. Partial Purification and Characterisation of Amylolytic Enzymes Obtained from Direct Fermentation of Sago Starch to Ethanol by Recombinant Yeast. Pakistan Journal of Biological Sciences, 4: 266-270.

DOI: 10.3923/pjbs.2001.266.270

URL: https://scialert.net/abstract/?doi=pjbs.2001.266.270

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