Expression of Bt Gene in a Dicot Plant Under Promoter Derived from a Monocot Plant


	Pakistan Journal of Biological Sciences
	Year: 2001 \| Volume: 4 \| Issue: 12 \| Page No.: 1518-1522 DOI: 10.3923/pjbs.2001.1518.1522

Expression of Bt Gene in a Dicot Plant Under Promoter Derived from a Monocot Plant

M. A. Khan , R. Makhdoom , T. Husnain , M. Z. Saleem , K. Malik , Z. Latif , I. Altosaar and S. Riazuddin

Abstract: The possibility of using a monocot (maize) derived ubiquitin (Ubi) promoter to express a fully modified insecticidal cry gene of Bacillus thuringiensis in dicot plant, tobacco was studied. Tobacco (Nicotiana tabacum cv. xanthi) plants were transformed with Bt gene Cry-1Ac driven by Ubi promoter. Transgenic plants were confirmed for transformation, gene expression and insecticidal activity through PCR, GUS assays, Southern blot, Western blot analyses and insect bioassays. Bioassays with cry-1Ac transformed T_o and T₁ transgenic plants showed high level of toxicity towards American bollworm (Heliothis armigera) giving 100% mortality of the larvae. This paper reported that monocot derived Ubi promoter expresses a Bt gene in a dicot plant in an effective manner to render the transformed plants highly resistant against Heliothis armigera.

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RELATED ARTICLES:

•	Occurrence of cry Genes in Bacillus thuringiensis (Bt) Isolates Recovered from Phylloplanes of Crops Growing in the New Delhi Region of India and Toxicity Towards Diamond-back Moth (Plutella xylostella)

•	Screening of Bacillus thuringiensis Isolates Recovered from Diverse Habitats in India for the Presence of cry1A-type Genes and Cloning of a cry1Ac33 Gene Toxic to Helicoverpa armigera (American Bollworm)

How to cite this article:

M. A. Khan , R. Makhdoom , T. Husnain , M. Z. Saleem , K. Malik , Z. Latif , I. Altosaar and S. Riazuddin , 2001. Expression of Bt Gene in a Dicot Plant Under Promoter Derived from a Monocot Plant. Pakistan Journal of Biological Sciences, 4: 1518-1522.

DOI: 10.3923/pjbs.2001.1518.1522

URL: https://scialert.net/abstract/?doi=pjbs.2001.1518.1522

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