Abstract:
The development of pure lines of maize by the application of tissue culture is considered to be more effective to enhance high yielding hybrids development. Somaclonal variations may be more useful for genetic material exchange in wide hybrids. Disease-free superior clones can be regenerated through tissue culture techniques, which can be applied to all known infectious agents but are especially valuable in elimination viruses and viroids from vegetatively propagated crops. Producing clean planting material through in vitromethods can significantly raise crop yield and quality. A synthetic medium is more useful than the natural products in the medium for the callus induction and growth. As the process of regeneration may be enhanced by adding synthetic plant hormones (auxins and cytokinines) which regulate cell division and differentiation.
Muhammad Ahsan, Syed Sadaqat Mehdi and Ihsan Khaliq, 2000. Tissue Culture and Breeding of Maize (Zee mays L.) "A Review". Pakistan Journal of Biological Sciences, 3: 1985-1988.
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