None of the 29 soybean cultivars and 40 germplasm lines tested were found immune to two isolates of soybean mosaic potyvirus (SMV-S1 and SMV-P1). Malakand-96 was the only cultivar found highly resistant to both S1 and P1 isolates. Swat-84, Bryan, Hobbit-87, Kingsay, Lugan, Sherman and Harper-87 were resistant to S1 and P1 whereas Rincondita was resistant to S1 and moderately resistant to P1 isolate. Similarly, Clark, Nare, NARC-V and Wahab-93 were resistant to P1 but not to S1 isolate. Wahab-93, Kharif-93, Ajmeri, Hodgson, Mid-Spray, Full-Walter, NARC-II, NARC-IV, NARC-VI, Clark and Nare were moderately resistant to S1 whereas Kharif93, Ajmeri, Hodgson, Full-Walter, NARC-Il, NARC-IV, Rincondita, Mid-Pharoah, NARC-VI and Winchester were moderately resistant to P1 isolate. William, Rawal-I, Mid-Pharoah, Bass, NARC-lll, NARC-I, NARC-V and Winchester were susceptible, to SMV-S1 whereas William, Rawal-I, Mid-Spray, Bass, NARC-I and NARC-III were susceptible to P1, Weber-84 was highly susceptible to both isolates of SMV. Among 40 soybean lines, GC-81083-63, GC-81084-51, GC-80072-2-6, AGS-253 and AVRDC-12, AVRDC-13 and AVRDC-15 were highly resistant to both isolates of SMV. Lines GC-81075-44, GC-81090-108, GC-81084-147, GC-81090-10, AGS-85, AGS-249, AVRDC-10, AGS-297, AVRDC-7 were resistant to S1 and P1 isolates whereas L-85-2308, AVRDC-5, AVRDC-14, were resistant to S1 and moderately resistant to P1 isolate. Line L-77-1863 was resistant to P1 but moderately resistant to S1 isolate. Similarly, GC-81080-13, GC-82117-8, AGS-154, L-88-8502, AVRDC-3, AVRDC-4, AVRDC-8 were moderately resistant to S1 and P1 isolates and AVRDC-11, GC-81084-134, GC-8108441, GC-81080-36, GC-81090-94, AVRDC-2, AVRDC-6 were moderately resistant to only P1 isolate. Ten soybean lines were susceptible and 3 highly susceptible to Si whereas 4 lines were susceptible and only one line was highly susceptible to P1 isolate.