
	Pakistan Journal of Biological Sciences
	Year: 2000 \| Volume: 3 \| Issue: 10 \| Page No.: 1716-1718 DOI: 10.3923/pjbs.2000.1716.1718

Production of Rabbit-antibuffalo Antibodies Horseradish Peroxidase Conjugate and Standardization of ELISA for Pasteurella multocida Antibodies

M. Anjum Zia, M. Yaqub, Khalil- ur-Rehman and Tariq Mahmood

Abstract: To standardize the indirect ELISA under local conditions, antispecies-antibodies were produced in rabbits. The antibodies against Pasteurella multocida were produced in buffalo calves by injecting the formalin, heat-killed organism_ These antibodies were inoculated into rabbits to produce rabbit-antibuffalo antibodies. These were isolated and partially purified by ammonium sulfate precipitation technique. Protein contents were estimated using Biuret method and the presence of rabbit-antibuffalo antibodies was confirmed and measured through agar gel precipitation test (AGPT). These isolated anti-antibodies were conjugated with horseradish peroxidase by two-step glutaraidehyde method. Ten mg of horseradish peroxidase was applied for the Anti-antibody-Enzyme Conjugate formation and the best results were recorded at 1 :100 and 1.200 dilutions of above conjugate when tested through indirect ELISA for H.S. anti-antibodies.

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How to cite this article:

M. Anjum Zia, M. Yaqub, Khalil- ur-Rehman and Tariq Mahmood, 2000. Production of Rabbit-antibuffalo Antibodies Horseradish Peroxidase Conjugate and Standardization of ELISA for Pasteurella multocida Antibodies. Pakistan Journal of Biological Sciences, 3: 1716-1718.

DOI: 10.3923/pjbs.2000.1716.1718

URL: https://scialert.net/abstract/?doi=pjbs.2000.1716.1718

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