Abstract:
A study of wall bound invertases in different tissues of mung bean cultivar has been undertaken. In young and mature leaves, acid as well as alkaline invertase activity was prominent. Senescent leaves showed the presence of alkaline invertase only. During flowering and fruiting, flowers and pods both posses acid invertase activity at initial stages of development whereas closed flower and mature pods showed the presence of alkaline invertase. Present results revealed that the young tissues of mung bean required acid invertase activity for their growth and development where hexoses were rapidly utilized with rapid sucrose hydrolysis. In mature and senescent tissues the appearance of alkaline invertase indicate the involvement of enzymes in the storage of sucrose. During present investigations, isozymes of invertases were studied by eluting them in sucrose solution of their respective pH optima after performing 7.5% polyacrylamide gel electrophoresis. Each tissue appears to possess wall bound invertases with predominance of a different activity in each case.
Simeen Mansoor and Farzana N. Naqvi, 2000. Changes in Wall Bound Invertase Activity in Young and Mature Tissues of Mung Bean (Vigna radiata). Pakistan Journal of Biological Sciences, 3: 1550-1552.
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