
	Pakistan Journal of Biological Sciences
	Year: 2000 \| Volume: 3 \| Issue: 9 \| Page No.: 1475-1479 DOI: 10.3923/pjbs.2000.1475.1479

Dual Luciferase Assay: An Efficient Method to Study the Polyadenylation Process

Amer Jamil, Muhammad Yaqub, Munir A. Sheikh and Harold G. Martinson

Abstract: Cleavage and polyadenylation is an obligatory step in mRNA biogenesis in eukaryotes. Poly(A) tails play an important role in mRNA turn over, transport and translation. Currently available methods for the study of polyadenylation are very cumbersome and involve radioactivity. Dual luciferase assay was found to be an efficient and equally reliable method to CAT assay and RNase protection assay. Comparison of these methods is reported in this paper.

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How to cite this article:

Amer Jamil, Muhammad Yaqub, Munir A. Sheikh and Harold G. Martinson, 2000. Dual Luciferase Assay: An Efficient Method to Study the Polyadenylation Process. Pakistan Journal of Biological Sciences, 3: 1475-1479.

DOI: 10.3923/pjbs.2000.1475.1479

URL: https://scialert.net/abstract/?doi=pjbs.2000.1475.1479

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