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Pakistan Journal of Biological Sciences
  Year: 2000 | Volume: 3 | Issue: 6 | Page No.: 1096-1097
DOI: 10.3923/pjbs.2000.1096.1097
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Hepatitis C Virus Core Protein Expression for Enzyme Linked Immunosorbant Assay

Khalid Pervez

HCV core region, being most conserved, in the whole genome can be used as a diagnostic marker in enzyme linked immuno-sorbant assay development. However, quasi species nature of HCV during its replication in host is the major hindrance in developing an effective therapeutic agent. Recombinant fusion protein for HCV immuno-assay from core region was prepared by PCR amplification and subsequent cloning in expression vector PET32a which was expressed by iso-propyl thio-B-galactosides (IPTG) in E. coli strain DE3 followed by purification by affinity chromatography. Fusion protein as an antigen reacted with human antisera containing antibodies. This HCV specific immuno-assay development based on recombinant HCV core protein will be the foundation in developing antiviral immuno-assays in Pakistan.
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How to cite this article:

Khalid Pervez , 2000. Hepatitis C Virus Core Protein Expression for Enzyme Linked Immunosorbant Assay. Pakistan Journal of Biological Sciences, 3: 1096-1097.

DOI: 10.3923/pjbs.2000.1096.1097






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