Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Pakistan Journal of Biological Sciences
  Year: 1999 | Volume: 2 | Issue: 2 | Page No.: 419-421
DOI: 10.3923/pjbs.1999.419.421
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Modified Splenic Cells Migration Inhibition Test For the Detection of Cell Mediated Immunity Against Coccidiosis in Chickens

Masood Akhtar , C.S. Hayat , M. Ashfaque , Iftikhar Hussain , Murad Ali Khan and Sultan Ayaz

Abstract:
A splenic cell migration inhibition test with modifications was successfully employed in the flat bottom micro titration plates to detect the cell mediated immunity more conveniently and accurately due to vaccine against coccidiosis. A drop of melted agarose (0.2% in PBS) was used to immobilize the splenic fragments. The splenic cells were culture in Hank`s Balanced salt containing fresh chicken plasma and maintained for 10 days. Twenty four hours after cultivation, dominant cell type migrated from a splenic fragment was leukocyte-like cells. At the fourth day these appeared predominantly large round cells and resembled to the macrophage. At the 10th day, spindle cells resembling of fibroblast appeared. The migration of the splenic cells from immunized chickens were inhibited remarkably with the antigen and there was a significant difference (P<0.01) in migration distance of the splenic cells with and without the antigen.
PDF Citation Report Citation
 RELATED ARTICLES:
  •    Delayed Hypersensitivity Reaction as a Measure of Cell Mediated Immunity in Chickens Vaccinated with Sonicated Coccidial Vaccine
How to cite this article:

Masood Akhtar , C.S. Hayat , M. Ashfaque , Iftikhar Hussain , Murad Ali Khan and Sultan Ayaz , 1999. Modified Splenic Cells Migration Inhibition Test For the Detection of Cell Mediated Immunity Against Coccidiosis in Chickens . Pakistan Journal of Biological Sciences, 2: 419-421.

DOI: 10.3923/pjbs.1999.419.421

URL: https://scialert.net/abstract/?doi=pjbs.1999.419.421

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 

Curve Bottom