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Pakistan Journal of Biological Sciences
  Year: 1999 | Volume: 2 | Issue: 4 | Page No.: 1432-1434
DOI: 10.3923/pjbs.1999.1432.1434
 
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In vitro Morphogenesis From Seeds of Helianthus Annuus L.

Aish Muhammed, Mustafa Sajid , Iqbal Hussain and Azra Quraishi

Abstract:
The objective of this study was to observe the in vitro morphogenetic response of a commercial hubrid SF-187 which is used in sunflower breeding programme. Basal MS media supplemented with NAA and BA was used. Cultures were incubated in complete obscurity at a temperature of 25°C. Morphogenesis and callogenesis were observed after 14 days of incubation. When exposed to light, shootlets turned green within two days. A maximum of fifty plants were regenerated from single explant when cultured on MS medium supplemented with 2.0 mg/l each of BAP and NAA. The plantlets were rooted when subcultured on MS media supplemented with 0.5 mg/l IAA.
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How to cite this article:

Aish Muhammed, Mustafa Sajid , Iqbal Hussain and Azra Quraishi , 1999. In vitro Morphogenesis From Seeds of Helianthus Annuus L.. Pakistan Journal of Biological Sciences, 2: 1432-1434.

DOI: 10.3923/pjbs.1999.1432.1434

URL: https://scialert.net/abstract/?doi=pjbs.1999.1432.1434

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