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Pakistan Journal of Biological Sciences
  Year: 1999 | Volume: 2 | Issue: 4 | Page No.: 1408-1411
DOI: 10.3923/pjbs.1999.1408.1411
 
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Nodulation, Symbiotic Growth and Yield of Vegetable Soybean Inoculated with Photorhizobium and Bradyrhizobium Zulkifli

H. Shamsuddin and S. B. Ang

Abstract:
A pot experiment with vegetable soybean (Glycine max L.) AGS190 inoculated with Photorhizobium was conducted with the following five treatments: three inoculation treatments with Photorhizobium (-N+Br) and a combination of both inocula (-N + Pr + Br), and two uninoculated nitrogen treatments, with and without inorganic nitrogen (+N-I, -N-I). The plants were harvested for symbiotic growth and yield after 42 and 65 days, respectively. The results showed that Photorhizobium was able to nodulate vegetable soybean; the nodules formed were larger (74mg/nodule) than the other two inoculation treatments involving Bradyrhizobium (32 and 35mg/nodule). The analysis N-solutes in xylem exudates indicated that nodules formed by Photorhizobium have high N2 fixing activity (98.3 %). However, a low concentration (0.19 %) of reducing sugar in the nodule tissues compared to the Bradyrhizobium and combined inoculum treatments (1.72 and 1.92 % respectively) lowered the total amount of N2 fixed; consequently, the relatively lower leaf N concentration (2.73 %) compared to control treatment with inorganic nitrogen (+N-I) (3.12%). A mixed inoculum of Photorhizobium and Bradyrhizobium showed a synergistic effect on the leaf N concentration (3.64 %) and pod yield (4.21 g/plant) compared to a single inoculation with Bradyrhizobium (3.31 %, 4.05 g/plant). Photorhizobium inoculation alone produced a significant reduction in pod yield (3.42g/plant) which corresponded directly to the lower leaf N concentration (2.73 %). This stud showed that Photorhizobium can nodulate vegetable soybean but have no positive effect in supplying photosynthate to the nodules and produced lower leaf N concentration and pod yield compared to Bradyrhizobium inoculation. co-inoculation with Photorhizobium and Bradyrhizobium produced a synergistic effect on the leaf N concentration and pod yield.
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How to cite this article:

H. Shamsuddin and S. B. Ang , 1999. Nodulation, Symbiotic Growth and Yield of Vegetable Soybean Inoculated with Photorhizobium and Bradyrhizobium Zulkifli. Pakistan Journal of Biological Sciences, 2: 1408-1411.

DOI: 10.3923/pjbs.1999.1408.1411

URL: https://scialert.net/abstract/?doi=pjbs.1999.1408.1411

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